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Doctor insights on: Elisa Or Western Blot Tests For Hiv

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Dr. Scott Keith
16 doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


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Is an HIV ELISA test always done with an western blot test?

Is an HIV ELISA test always done with an western blot test?

No: In general, the elisa is done first, and the lab then does a western blot if the elisa is positive. However, it is possible to order just the western blot, in which case the elisa usually isn't done. ...Read more

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Can an HIV antibody screening test be positive but the western blot negative?

Can an HIV antibody screening test be positive but the western blot negative?

Yes: There are 2 reasons for this kind of result: (1) a false positive, or (2) early infection before seroconversion. In someone at low risk for HIV without symptoms, another negative WB in 1 month means there's no infection. In someone at risk or with symptoms of acute infection, a viral load can be helpful. The new 4th generation antigen/antibody tests will eliminate this problem. ...Read more

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Does the western blot trump the elisa? Tested positive via ELISA IgG for hsv2,but later tested negative via western blot. No symptoms. Do I have hsv2?

Western blot more: specific, ELISA more sensitive. One positive ELISA and one negative Western blot says that you do not have enough antibodies to HSV 2 for confirmation of diagnosis. It may be prudent to repeat the tests in 3-4 months to make sure that negative Western blot was not due to incomplete/early immune response. ...Read more

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Tested false positive ELISA HIV test last month. Negative ifa and western blot and naat. 2 negative oraquick all 1 1/2 year post exposure. Hiv?

Negative: If nat was negative at 6 months after exposure you not likely to have hiv. Given all the negative test results, you should not worry about the one apparently false positive. ...Read more

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How reliable is a HIV ELISA test vs a western blot?

HIV testing: Elisa assay is quite sensitive (will detect antibodies best) but the western blot is more specific and confirm the results of the elisa if it was positive, as being either truly positive or false-positive. There are several other circumstances in which conditions other than HIV will produce a positive elisa assay. If there are symptoms and too early for antibody test, then PCR for RNA is best. ...Read more

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Which one is better cmia or ELISA for HCV testing? Tested positive for HCV by ELISA and negative by cmia.

Which one is better cmia or ELISA for HCV testing? Tested positive for HCV by ELISA and negative by cmia.

False positive Hep C: When hepatitis antibody is positive but confirmatory tesr or hepc viral load is undetectable it is presumed to be false positive. Could occur in conditions with excessive antibodies. ...Read more

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Can I take 2 western blot HIV tests?

Yes: Of course the lab would be happy to accept your money for repeat testing - but the question is why a repeat western blot. If the elisa antibody screen is negative you do not need western blot confirmation, and if it is positive but the western blot results suggested biological false-positive results, then if you do not believe these, a PCR for viral nucleic acid is the best answer. ...Read more

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2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!

2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!

Likely negative: There is typically a "window" of 3-6 months after exposure where you could be HIV positive but test negative. Since you didn't get your testing until well after that, i'd say the confirmatory tests (western blot, ifa) are what you should go by. The elisa is very sensitive and can have false positives- that's why we rely on the confirmatory tests. I would consider your status to be negative. ...Read more

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Can negative test results be trusted for a prostitute with HIV antibody rapid test and HIV duo test?

Can negative test results be trusted for a prostitute with HIV antibody rapid test and HIV duo test?

Not necessarily: If the person just acquired the infection, she or he may be carrying the virus that will not be detected by either antibody or antigen tests. Testing for viral nucleic acid is the earliest way to detect HIV infection. ...Read more

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Can CMV virus and type 2 diabetes together make the HIV western blot and PCR viral load tests false positive for hiv?

Can CMV virus and type 2 diabetes together make the HIV western blot and PCR viral load tests false positive for hiv?

Yes: A false positive is possible. A test done by a reputable lab is far better than the otc options! false positives can happen even if your health status is perfect! ...Read more

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With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago i received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t

With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago i received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t

False-positives: Screening tests are designed to be highly sensitive so they don't miss anyone with the disease. However, they can be so sensitive that they occasionally give a false-positive result. That is, the test appears positive for someone without the disease. The western blot, being time consuming and extra cost, is only used to make sure a positive is really a positive. Talk with your doc for more info. ...Read more

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Hiv western blot- all antibodies -ve except 1p17 detected. Hiv tridot is negative.What is further advise?

HIV: Hiv western blot confirmation is generally not necessary with some of the newer serological tests available. The best measure is a pcr for HIV rna. This is expensive, but definitive when negative. ...Read more

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Is RNA PCR test for HIV accurate?

Is RNA PCR test for HIV accurate?

Yes: The nucleic acid test for HIV becomes positive about 20 days after exposure to the virus. ...Read more

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Are Lyme tests Elisa & western blot actually unreliable half the time like holistic/naturopath doctors or LLMD's say?

Not true: Naturopaths spend a great deal of time and effort promoting themselves by debunking conventional medicine. But who do you think they go to when they are sick? The tests you mention are mostly reliable but there is a small margin of error for any tests. To say these are unreliable at the 50% level is marketing and untrue. ...Read more

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Are false positive HIV ELISA tests a sign of HSV 2?

Are false positive HIV ELISA tests a sign of HSV 2?

Please get help: Please call your therapist. You obviously do not believe anything we tell you here. Don't understand why you think it is okay to take our time up when you don't believe our answers are accurate. Please find another forum to deal with your internal struggles. ...Read more

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Tested positive for Lyme disease IgM but my western blot test came back negtive?

Tested positive for Lyme disease IgM but my western blot test came back negtive?

Then it's not Lyme: Lyme disease can be tricky -and you want to make sure you see someone like an Infectious Diseases doctor who knows how to treat it. The diagnosis is actually based on clinical findings, and the lab testing is generally 'supportive'. Since you're Western blot is negative, then it's very unlikely you have Lyme. There are other diseases that could cause 'Lyme-like' symptoms -and good to get evaluated ...Read more

Dr. Ed Friedlander
1,489 doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more


Elisa (Definition)

Enzyme-linked immunosorbent assay (elisa) is a test that uses antibodies and color change ...Read more