Doctor insights on:
Elisa Or Western Blot Tests For Hiv
Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more
Yes: There are 2 reasons for this kind of result: (1) a false positive, or (2) early infection before seroconversion. In someone at low risk for HIV without symptoms, another negative WB in 1 month means there's no infection. In someone at risk or with symptoms of acute infection, a viral load can be helpful. The new 4th generation antigen/antibody tests will eliminate this problem. ...Read more
Does the western blot trump the elisa? Tested positive via ELISA IgG for hsv2,but later tested negative via western blot. No symptoms. Do I have hsv2?
Western blot more: specific, ELISA more sensitive. One positive ELISA and one negative Western blot says that you do not have enough antibodies to HSV 2 for confirmation of diagnosis. It may be prudent to repeat the tests in 3-4 months to make sure that negative Western blot was not due to incomplete/early immune response. ...Read more
HIV testing: Elisa assay is quite sensitive (will detect antibodies best) but the western blot is more specific and confirm the results of the elisa if it was positive, as being either truly positive or false-positive. There are several other circumstances in which conditions other than HIV will produce a positive elisa assay. If there are symptoms and too early for antibody test, then PCR for RNA is best. ...Read more
After exposure, 3months HIV quali RNA test negative, not detected.4.5months HIV quick test and ELISA test negative.Are those tests all exclusive?
Which one is better cmia or ELISA for HCV testing? Tested positive for HCV by ELISA and negative by cmia.
Yes: Of course the lab would be happy to accept your money for repeat testing - but the question is why a repeat western blot. If the elisa antibody screen is negative you do not need western blot confirmation, and if it is positive but the western blot results suggested biological false-positive results, then if you do not believe these, a PCR for viral nucleic acid is the best answer. ...Read more
2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!
Likely negative: There is typically a "window" of 3-6 months after exposure where you could be HIV positive but test negative. Since you didn't get your testing until well after that, i'd say the confirmatory tests (western blot, ifa) are what you should go by. The elisa is very sensitive and can have false positives- that's why we rely on the confirmatory tests. I would consider your status to be negative. ...Read more
Can negative test results be trusted for a prostitute with HIV antibody rapid test and HIV duo test?
Can CMV virus and type 2 diabetes together make the HIV western blot and PCR viral load tests false positive for hiv?
With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago i received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t
False-positives: Screening tests are designed to be highly sensitive so they don't miss anyone with the disease. However, they can be so sensitive that they occasionally give a false-positive result. That is, the test appears positive for someone without the disease. The western blot, being time consuming and extra cost, is only used to make sure a positive is really a positive. Talk with your doc for more info. ...Read moreSee 1 more doctor answer
Are Lyme tests Elisa & western blot actually unreliable half the time like holistic/naturopath doctors or LLMD's say?
Not true: Naturopaths spend a great deal of time and effort promoting themselves by debunking conventional medicine. But who do you think they go to when they are sick? The tests you mention are mostly reliable but there is a small margin of error for any tests. To say these are unreliable at the 50% level is marketing and untrue. ...Read moreSee 2 more doctor answers
No: no effect on testing.Get a more detailed answer ›
Then it's not Lyme: Lyme disease can be tricky -and you want to make sure you see someone like an Infectious Diseases doctor who knows how to treat it. The diagnosis is actually based on clinical findings, and the lab testing is generally 'supportive'. Since you're Western blot is negative, then it's very unlikely you have Lyme. There are other diseases that could cause 'Lyme-like' symptoms -and good to get evaluated ...Read more
I am wondering about HIV results. I have tested at 5 months post exposure with dna/pcr, antibody, western blot test (negative). Is this conclusive?
Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids),  a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more
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